agkistrodon acutus meaning in English
白花蛇
Examples
- It is shown that the obtained proteins ( hq - 5 & tsk - 2 ) can be identified as novel components of anti - coagulation enzyme family in the venom of agkistrodon acutus by the analysis of the n - terminal sequence of tsk - 2
其一级序列为rtefqrymeivv 。与从蕲蛇蛇毒中分离出的其它组分相比， hq - 5及其降解产生的组分( tsk - 2 )为新的抗凝血组分。 - Method was investigated to extract multiple proteins from complex system including water - soluble fraction of egg yolk and agkistrodon acutus venom by bacterial specific absorption . immunoglobulin yolk ( igy ) were absorbed directly from water - soluble fraction by streptococcus mutans , then eluted and further purified by columns of thiophilic gel . based on above experiments of antibody extraction , bacterium cells were applied further to extract interactive proteins from crude venom of agkistrodon acutus
首先，利用变异链球菌( s . mutans )与其特异性抗体的吸附作用从水溶性蛋白组分中分离出卵黄抗体( igy ) ，并将菌体上洗脱下来的igy用嗜硫色谱分离纯化，得到高纯度的细菌特异性抗体。 - The cdna sequence with 789 bp encodes an uncompleted open reading frame ( orf ) of 263 amino acids , which included a signal peptide of 18 amino acids , a proposed propeptide of 171 amino acids , a partial mature protein of 74 amino acids . the sequences of signal peptide and propeptide are very similar to ones from wannan agkistrodon acutus reported by fan chunyan , but it only contains partial amino acids of mature protein . the 800 bp is likely to be a part of gene encoding metalloproteinase agkistrodon acutus from guangxi province
结论： 600bp产物和已报道的皖南五步蛇纤溶酶金属蛋白酶氨基酸序列相比较，有90 . 6 %的同源性； 800bp产物中信号肽及前肽和已报道的皖南五步蛇金属蛋白酶很相似，同源性为97 . 9 % ，但仅含有部分金属蛋白酶成熟肽的氨基酸序列，推测此片段为广< wp = 6 >西五步蛇金属蛋白酶原基因的一部分。 - The 600 bp and 800 bp pcr products were cloned into the pgem - t easy vector . their cdna sequences were determined with positive clones or purified pcr product . conclusion : compared the 600 bp pcr product with the amino acids sequence for the fibrinolysin metalloproteinase from the venom of agkistrodon acutus from the southern of anhui province , their homology is 90 . 6 %
结果：其中一对引物扩增得到一600bp产物；另一对引物扩增得到三条特异性的dna条带，大小分别为1 . 5kb 、 1 . 3kb和800bp ，将600bp和800bpdna进行克隆及测序，并推导编码的氨基酸序列。 - Objective : to clone and sequence the cdna encoding metalloproteinase from the venom of agkistrodon acutus from northen mountain area of guangxi province . methods : one step method was used to extract total rna from the venom of agkistrodon acutus found in northern mountain area of guangxi province . different kinds of cdna encoding metalloproteinase were amplified by one step method ( rt - pcr and pcr reactions occurred in the same tube ) using different primers
方法：从桂北五步蛇毒腺中抽提总rna ，利用不同的引物，采用一步法（ rt - pcr和pcr在同一管内进行）扩增出不同的dna条带，利用平端连接的方法将pcr扩增产物克隆至pgem - teasy载体，转化大肠杆菌jm109 ，挑选白色菌落提取质粒，用pcr对其进行鉴定，直接利用纯化pcr产物或提取阳性菌落质粒进行测序。